Extraction & Isolation Techniques
The second phase of phytopharmaceutical research converts authenticated plant material into a phytochemically meaningful extract ready for screening and standardisation. Because extraction efficiency and selectivity determine which phytoconstituents survive into downstream analysis, this phase covers the underlying principles of extraction, conventional and modern green extraction technologies, and the criteria for solvent selection in herbal extraction. It then addresses the isolation and purification of phytoconstituents through column chromatography, flash chromatography, and preparative HPLC, before concluding with marker compound isolation, yield optimisation and extract characterisation, and residual solvent and green chemistry reporting.
Chapter overview
The objectives, principles, and extraction-to-isolation workflow that underpin the recovery and purification of phytoconstituents from authenticated plant material.
01
Objectives
02
Principles of Extraction
03
Conventional Extraction Methods
04
Modern and Green Extraction Technologies
05
Solvent Selection in Herbal Extraction
06
Isolation and Purification of Phytoconstituents
07
Column Chromatography
08
Flash Chromatography and Preparative HPLC
09
Marker Compound Isolation
10
Yield Optimisation and Extract Characterisation