Biological activity evaluation is the process by which the phytochemical composition data generated in Phase 3 is translated into functional pharmacological evidence of therapeutic potential, following a deliberately hierarchical, resource-efficient strategy that progresses from in-vitro screening through in-vivo confirmation to mechanistic study. This phase examines the antioxidant, antimicrobial, anti-inflammatory, anticancer, hepatoprotective, nephroprotective, and immunomodulatory assay platforms used to evaluate herbal candidates, concluding with the toxicological evaluation required to establish candidate safety.
The objectives, evaluation strategy, and pharmacological assay platforms that underpin biological activity evaluation of herbal candidates.